Abstract: A new method for rational design of molecularly imprinted polymer(MIP) of 4-nitrophenol(4-NP) was established. Using the method, nonimprinted polymer(NIP) library screening, vinylimidazole was selected as the best functional monomer. Acetonitrile and trimethylolpropane trimethacrylate(TRIM) were the best polymerization solvent and crosslinker, respectively. Through the optimization of the solid phase extraction(SPE) profile, the best imprinting effect was achieved in pH=2.5 phosphate buffer, washing with the buffer containing 45% acetonitrile and eluting with methanol. The maximum adsorption capacity of the polymer was 5.8 mg/g. Extraction recoveries of imprinted polymers for 4-NP and its analogs, phenol and 4-chlorophenol, were found to be 96.0%, 78.8% and 77.8%, respectively, proving its high selectivity. It was also successfully used for SPE of 4-NP from spiked tap water with a recovery of 93.1%. The method is proved to be effective and fast for the preparation of MIPs.
Template and target information: 4-nitrophenol, 4-NP
Author keywords: molecularly imprinted polymer, solid phase extraction, nitrophenol, Monomer screening, Dummy-template