MIPs logo MIPdatabase      MIP2024 Conference banner, website is now open, register on site for important updates   
Custom Search
Reference type: Journal
Authors: Dechtrirat D, Gajovic-Eichelmann N, Bier FF, Scheller FW
Article Title: Hybrid Material for Protein Sensing Based on Electrosynthesized MIP on a Mannose Terminated Self-Assembled Monolayer.
Publication date: 2014
Journal: Advanced Functional Materials
Volume: 24
Issue: (15)
Page numbers: 2233-2239.
DOI: 10.1002/adfm.201303148

Abstract: A novel strategy to prepare a surface confined molecularly imprinted polymer (MIP) film directly on a transducer surface for protein sensing is achieved by combining interaction with a natural binding receptor and binding to a fully synthetic MIP. A thiolated oligoethyleneglycol (OEG)/mannose conjugate is first self-assembled on the transducer surface. Then the carbohydrate binding protein, concanavalin A (ConA), is "vectorially" immobilized as a submonolayer on the underlying mannose modified surface. Afterwards, an ultrathin polyscopoletin film with the thickness comparable to that of the protein is electrodeposited on the top. This architecture ensures that the target is confined to the film surface. The resulting functional material shows an approximately 20-fold higher affinity than that obtained from the mannose self-assembled monolayer. This result shows a synergism between multivalent binding of the natural sugar ligand and the non-covalent interactions of the target within the MIP cavities. Recognition capability of the film is characterized by a real-time measurement using quartz crystal microbalance. In comparison to the non-imprinted film, the imprinted film reveals 8.6 times higher binding capacity towards ConA. High discrimination towards the target protein's homologues shows size and shape specificity of the imprint
Template and target information: protein, concanavalin A, ConA
Author keywords: electropolymerization, molecular imprinting, molecular recognition, self-assembly, ultrathin films

  I love MISPE mug  multi MIPs logo mug  British periodic table patriotic tote bag


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner