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Reference type: Journal
Authors: Du XJ, Zhang F, Zhang HX, Wen YJ, Saren TY
Article Title: Substitution of antibody with molecularly imprinted 96-well plate in chemiluminescence enzyme immunoassay for the determination of chloramphenicol residues.
Publication date: 2014
Journal: Food and Agricultural Immunology
Volume: 25
Issue: (3)
Page numbers: 411-422.
DOI: 10.1080/09540105.2013.821598

Abstract: A direct competitive chemiluminescence enzyme immunoassay was developed by using the molecularly imprinted 96-well plate as an artificial antibody to detect chloramphenicol (CAP). The artificial antibody was synthesised on the well surface of MaxiSorp polystyrene 96-well plate and CAP was conjugated with the horseradish peroxidase. The results showed that the imprinted plate exhibited antibody-like binding ability. The plate showed fast adsorption rate, 66% adsorption was finished within 20 min. The cross-reactivity for CAP, florfenicol and thiamphenicol were 100%, 1.25% and 2.08%, respectively. And the imprinted plate could be reused for many times without loss of sensitivity. The IC50 and the detection limit values under optimum conditions were 30 2 μg L-1 and 0.9 0.01 μg L-1, respectively. The plate was used to detect CAP in sea cucumber, which showed excellent recoveries ranging from 89% to 98.7%. And the result correlated well with that obtained by the CAP enzyme-linked immunosorbent assay (ELISA) kit
Template and target information: chloramphenicol, CAP
Author keywords: chloramphenicol, chemiluminescence enzyme immunoassay, molecularly imprinted 96-well plate, artificial antibody

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