MIPs logo MIPdatabase     MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Du XJ, Zhang F, Zhang HX, Wen YJ, Saren TY
Article Title: Substitution of antibody with molecularly imprinted 96-well plate in chemiluminescence enzyme immunoassay for the determination of chloramphenicol residues.
Publication date: 2014
Journal: Food and Agricultural Immunology
Volume: 25
Issue: (3)
Page numbers: 411-422.
DOI: 10.1080/09540105.2013.821598

Abstract: A direct competitive chemiluminescence enzyme immunoassay was developed by using the molecularly imprinted 96-well plate as an artificial antibody to detect chloramphenicol (CAP). The artificial antibody was synthesised on the well surface of MaxiSorp polystyrene 96-well plate and CAP was conjugated with the horseradish peroxidase. The results showed that the imprinted plate exhibited antibody-like binding ability. The plate showed fast adsorption rate, 66% adsorption was finished within 20 min. The cross-reactivity for CAP, florfenicol and thiamphenicol were 100%, 1.25% and 2.08%, respectively. And the imprinted plate could be reused for many times without loss of sensitivity. The IC50 and the detection limit values under optimum conditions were 30 2 μg L-1 and 0.9 0.01 μg L-1, respectively. The plate was used to detect CAP in sea cucumber, which showed excellent recoveries ranging from 89% to 98.7%. And the result correlated well with that obtained by the CAP enzyme-linked immunosorbent assay (ELISA) kit
Template and target information: chloramphenicol, CAP
Author keywords: chloramphenicol, chemiluminescence enzyme immunoassay, molecularly imprinted 96-well plate, artificial antibody


  mipdatabase.com logo imprinters do it in bulk shirt  Science Teacher peptide mug  Perpetual student shirt

Molecules Special Issue call     Sensors Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon