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Reference type: Journal
Authors: Tan L, Huang C, Peng RF, Tang YW, Li WM
Article Title: Development of hybrid organic-inorganic surface imprinted Mn-doped ZnS QDs and their application as a sensing material for target proteins.
Publication date: 2014
Journal: Biosensors and Bioelectronics
Volume: 61
Page numbers: 506-511.
DOI: 10.1016/j.bios.2014.06.004
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566314004072

Abstract: Applying molecular imprinting techniques to the surface of functionalized quantum dots (QDs) allows the preparation of molecularly imprinted polymers (MIPs) with accessible, surface exposed binding sites and excellent optical properties. This paper demonstrates a new strategy for producing such hybrid organicGă˘inorganic imprinted Mn-doped ZnS QDs for specific recognition of bovine hemoglobin. The technique provides surface grafting imprinting in aqueous solutions using amino modified Mn-doped ZnS QDs as supports, acrylamide and methacrylic acid as functional monomers, γ-methacryloxypropyl trimethoxy silane as the grafting agent, and bovine hemoglobin as a template. The amino propyl functional monomer layer directs the selective occurrence of imprinting polymerization at the QDs surface through copolymerization of grafting agents with functional monomers, but also acts as an assistive monomer to drive the template into the formed polymer shells to create effective recognition sites. Using MIP-QDs composites as a fluorescence sensing material, trace amounts of bovine hemoglobin are signaled with high selectivity by emission intensity changes of Mn-doped ZnS QDs, which is embedded into the imprinted polymers
Template and target information: protein, bovine hemoglobin
Author keywords: molecularly imprinted, Hybrid composite material, Quantum dots, Fluorescence probes


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