Abstract: Objective A ractopamine (RCT) imprinted polymer was prepared as a recognition element, which had the ability of specific recognition. Based on the recognition element, a novel microfluidic chip-chemiluminescence(CL) sensor was designed and assembled to monitor the RCT content in food. Methods The RCT imprinted polymer was synthesized by precipitation polymerization. SEM, FT-IR spectra and absorption experiment were applied as characterization methods. With MIPs working as the recognition element, microfluidic chip as flow reaction cell, a novel flow analysis CL sensor for the determination of RCT was developed and optimized. Results Under the optimal conditions, the relative CL intensity showed a linear relationship in the range of 6 - 960 ng/mL RCT with detection limit of 0.83 ng/mL. The linear regression equation was Δ I = 4.8777C -9.7851, r = 0.9907. The relative standard deviation was 7.1% for 10 ng/ mL RCT (n=11). The method was successfully used to analyze the RCT content in pig liver and beef. The recovery was between 90.3% and 99.8%. Conclusion The method is rapid, accurate, and simple pre-treatment, which can be well used in the determination of ractopamine residues in food.
Template and target information: ractopamine
Author keywords: Ractopamine, Molecularly imprinted polymers, microfluidic chip chemiluminescence sensor