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Reference type: Journal
Authors: Wan DH, Wang XP, Wu ZB, Tang H, Liu XY
Article Title: A microfluidic chip-chemiluminescence sensor based on molecular imprinted recognition for determination of ractopamine.
Publication date: 2014
Journal: Journal of Food Safety and Quality
Volume: 5
Issue: (5)
Page numbers: 1391-1397.
Alternative URL: http://www.chinafoodj.com/ch/reader/view_abstract.aspx?file_no=20140413001&flag=1

Abstract: Objective A ractopamine (RCT) imprinted polymer was prepared as a recognition element, which had the ability of specific recognition. Based on the recognition element, a novel microfluidic chip-chemiluminescence(CL) sensor was designed and assembled to monitor the RCT content in food. Methods The RCT imprinted polymer was synthesized by precipitation polymerization. SEM, FT-IR spectra and absorption experiment were applied as characterization methods. With MIPs working as the recognition element, microfluidic chip as flow reaction cell, a novel flow analysis CL sensor for the determination of RCT was developed and optimized. Results Under the optimal conditions, the relative CL intensity showed a linear relationship in the range of 6 - 960 ng/mL RCT with detection limit of 0.83 ng/mL. The linear regression equation was Δ I = 4.8777C -9.7851, r = 0.9907. The relative standard deviation was 7.1% for 10 ng/ mL RCT (n=11). The method was successfully used to analyze the RCT content in pig liver and beef. The recovery was between 90.3% and 99.8%. Conclusion The method is rapid, accurate, and simple pre-treatment, which can be well used in the determination of ractopamine residues in food.
Template and target information: ractopamine
Author keywords: Ractopamine, Molecularly imprinted polymers, microfluidic chip chemiluminescence sensor


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