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Reference type: Journal
Authors: Ertürk G, Berillo D, Hedström M, Mattiasson B
Article Title: Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA.
Publication date: 2014
Journal: Biotechnology Reports
Volume: 3
Page numbers: 65-72.
DOI: 10.1016/j.btre.2014.06.006
Alternative URL: http://www.sciencedirect.com/science/article/pii/S2215017X14000198

Abstract: An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 x 10-20 - 1.0 x 10-8 M with a limit of detection (LOD) of 1.0 x 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications
Template and target information: protein, bovine serum albumin, BSA
Author keywords: Microcontact imprinting, Capacitive biosensor, BSA detection


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