MIPs logo MIPdatabase     MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Song RY, Hu XL, Guan P, Li J, Zhao N, Wang QL
Article Title: Molecularly imprinted solid-phase extraction of glutathione from urine samples.
Publication date: 2014
Journal: Materials Science and Engineering: C
Volume: 44
Page numbers: 69-75.
DOI: 10.1016/j.msec.2014.08.005
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0928493114004901

Abstract: Molecularly imprinted polymer (MIP) particles for glutathione were synthesized through iniferter-controlled living radical precipitation polymerization (IRPP) under ultraviolet radiation at ambient temperature. Static adsorption, solid-phase extraction, and high-performance liquid chromatography were carried out to evaluate the adsorption properties and selective recognition characteristics of the polymers for glutathione and its structural analogs. The obtained IRPP-MIP particles exhibited a regularly spherical shape, rapid binding kinetics, high imprinting factor, and high selectivity compared with the MIP particles prepared using traditional free-radical precipitation polymerization. The selective separation and enrichment of glutathione from the mixture of glycyl-glycine and glutathione disulfide could be achieved on the IRPP-MIP cartridge. The recoveries of glutathione, glycyl-glycine, and glutathione disulfide were 95.6% 3.65%, 29.5% 1.26%, and 49.9% 1.71%, respectively. The detection limit (S/N = 3) of glutathione was 0.5 mg L-1. The relative standard deviations (RSDs) for 10 replicate detections of 50 mg L-1 of glutathione were 5.76%, and the linear range of the calibration curve was 0.5 mg L-1 to 200 mg L-1 under optimized conditions. The proposed approach was successfully applied to determine glutathione in spiked human urine samples with recoveries of 90.24% to 96.20% and RSDs of 0.48% to 5.67%
Template and target information: glutathione
Author keywords: Glutathione, molecularly imprinted polymer, living radical polymerization, Selectivity extraction, Solid-phase extraction


  I love MIPs hat  multi MIPs logo mug  America periodic table shirt

Molecules Special Issue call     Sensors Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon