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Reference type: Journal
Authors: Thongchai W, Liawruangrath B, Greenway GM
Article Title: Separation and determination of gallic acid and ellagic acid by high performance liquid chromatographic method.
Publication date: 2014
Journal: Burapha University's Journal Online
Volume: 19
Issue: (3)
Page numbers: 129-140.
Alternative URL: http://digital_collect.lib.buu.ac.th/ojs/index.php/science/article/view/3225

Abstract: Molecularly imprinted polymers stationary phase was prepared for the separation of gallic acid (GA) and ellagic acid (EGA) by thermal polymerization method using benzoylperoxide as initiator, the mixtures of dodecanol and toluene as porogens, divinylbenzene as cross-linker and styrene as a functional monomer, respectively. Gallic acid and ellagic acid solutions were separated by HPLC using GA-EGA-MIP column, 50 x 4.6 mm as MIP column with the mobile phase; water-acetonitrile (5:95, v/v) adjusted to pH 3.0. The flow rate was adjusted to 0.1 mL min-1. The absorption was made at 280 nm. The sample solution was collected from GA-EGA-MIP column and then was injected into the analytical column used was Ultra C18 column, 150 x 4.6 mm for determination of gallic acid and ellagic acid in sample solution. The mobile phase was eluted with gradient system containing a mixture of acetonitrile and water. The flow rate was used to 1.0 mL min-1. The injection volume was adjusted to 10 μL and the absorption was made at 280 nm. The results of quantitation limit of GA and EGA were found to be 1.02 and 2.29 μg mL-1, respectively. This approach method is shown to be successfully for the separation and purification of gallic acid and ellagic acid from Dimocarpus longan extract
Template and target information: gallic acid, GA, ellagic acid, EGA
Author keywords: Molecularly imprinted polymers, high performance liquid chromatography, Gallic acid, Ellagic acid, Dimocarpus longan

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