Abstract: It takes about 10 days to detect the bacterium in water and food using traditional methods and there is an essential need for the introduction and manufacturing of nano biosensor kits for a fast detection in few minutes, so an attempt in determining the mass of Salmonella typhi by means of a sensor design was made to obtain this shortened. In this research, by means of a micro contact imprinted polymer scheme, molecularly imprinted polymer was achieved. First, Meta acrylic acid (MAA) with covalent bond among its monomers formed a white polymer and hydrogen bond between antibody and MAA. They were used to prepare imprinted molecule and polymer. It was noticed that with the aid of fluorescence converter and its connection with the antibody of Salmonella typhi bacterium, it would be possible to detect the Salmonella typhi antigen. The results indicate that detecting the concentration of Salmonella typhi bacterium is possible with a minimum mass of 10 colonies per ml in polluted waters. The achieved results were quantified by a spectrofluorometer device. Additionally,the Ecoli bacteria were inseminated in an aquatic environment containing Salmonella typhi and it was detected that there was no interference with function of sensor. Besides, sensitivity of the sensor up to 60 days was examined and it was agreed that performance of the sensor could be verified up to 56 days and then would start to diminish. In conclusion the role of sensor at 10-1 to 10^9 concentration of Salmonella typhi antigen are exclusively precise and sensitive.
Template and target information: Salmonella typhi antigen
Author keywords: Biological nano-sensor, Microcontact, Salmonella Typhi, Spectrofluorimetery