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Abstract: New composite layer architecture of 3D hydrogel polymer network that is loaded with molecularly imprinted polymer nanoparticles (nanoMIP) is reported for direct optical detection of low-molecular-weight compounds. This composite layer is attached to the metallic surface of a surface plasmon resonance (SPR) sensor in order to simultaneously serve as an optical waveguide and large capacity binding-matrix for imprinted target analyte. Optical waveguide spectroscopy (OWS) is used as a label-free readout method allowing direct measurement of refractive index changes that are associated with molecular binding events inside the matrix. This approach is implemented by using a photo-crosslinkable poly(N-isopropylacrylamide)-based hydrogel and poly[(ethylene glycol dimethylacrylate)-(methacrylic acid)] nanoparticles that are imprinted with l-Boc-phenylalanine-anilide (l-BFA, molecular weight 353 g mol-1). Titration experiments with the specific target and other structurally similar reference compounds show good specificity and limit of detection for target l-BFA as low as 2 x 10-6 M
Template and target information: l-Boc-phenylalanine-anilide, l-BFA
Author keywords: label-free biosensors, hydrogels, Molecularly imprinted polymers, nanoparticles, optical waveguides spectroscopy