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Reference type: Journal
Authors: Lan F, Ma SH, Yang Q, Xie LQ, Wu Y, Gu ZW
Article Title: Polydopamine-based superparamagnetic molecularly imprinted polymer nanospheres for efficient protein recognition.
Publication date: 2014
Journal: Colloids and Surfaces B: Biointerfaces
Volume: 123
Page numbers: 213-218.
DOI: 10.1016/j.colsurfb.2014.09.018
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0927776514004895

Abstract: A new strategy for synthesis of superparamagnetic molecularly imprinted polymer nanospheres (MIPNSs) for efficient protein recognition is described here. Homogeneous hydroxyl group functionalized Fe3O4/polymethyl methacrylate (PMMA) composite nanospheres were prepared using improved miniemulsion polymerization. Uniform superparamagnetic MIPNSs were obtained via self-polymerization of dopamine (DA) on the surface of Fe3O4/PMMA composite nanospheres in the presence of lysozyme (lyz) template. The as-synthesized Fe3O4/PMMA/PDA MIPNSs had average diameters of 180 nm, high saturation magnetization and a good magnetic response. The lyz-imprinted Fe3O4/PMMA/PDA MIPNSs exhibited specific recognition and efficient adsorption capacity toward lyz template. The amount of lyz adsorbed onto the lyz-imprinted Fe3O4/PMMA/PDA MIPNSs was about 4 times greater than that of the Fe3O4/PMMA/PDA non-imprinted polymer nanospheres (NIPNSs) and about 14, 5, and 5 times greater than that of BSA, BHb, and cyt C, respectively
Template and target information: protein, lysozyme, lyz
Author keywords: superparamagnetic, Molecularly imprinted polymer nanospheres (MIPNSs), Polymethyl methacrylate (PMMA), Polydopamine, protein recognition


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