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Reference type: Journal
Authors: Li QR, Yang KG, Liang Y, Jiang B, Liu JX, Zhang LH, Liang Z, Zhang YK
Article Title: Surface Protein Imprinted Core-Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition-Fragmentation Chain Transfer Strategy.
Publication date: 2014
Journal: ACS Applied Materials & Interfaces
Volume: 6
Issue: (24)
Page numbers: 21954-21960.
DOI: 10.1021/am5072783

Abstract: A novel kind of lysozyme (Lys) surface imprinted core-shell particles was synthesized by reversible addition-fragmentation chain transfer (RAFT) strategy. With controllable polymer shell chain length, such particles showed obviously improved selectivity for protein recognition. After the RAFT initial agent and template protein was absorbed on silica particles, the prepolymerization solution, with methacrylic acid and 2-hydroxyethyl methacrylate as the monomers, and N,N'-methylenebis(acrylamide) as the cross-linker, was mixed with the silica particles, and the polymerization was performed at 40 C in aqueous phase through the oxidation-reduction initiation. Ater polymerization, with the template protein removal and destroying dithioester groups with hexylamine, the surface Lyz imprinted particles were obtained with controllable polymer chain length. The binding capacity of the Lys imprinted particles could reach 5.6 mg protein/g material, with the imprinting factor (IF) as 3.7, whereas the IF of the control material prepared without RAFT strategy was only 1.6. The absorption equilibrium could be achieved within 60 min. Moreover, Lys could be selectively recognized by the imprinted particles from both a four-proteins mixture and egg white sample. All these results demonstrated that these particles prepared by RAFT strategy are promising to achieve the protein recognition with high selectivity
Template and target information: protein, lysozyme, Lys

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