Abstract: The chloramphenicol imprinted polymers were prepared by microwave-assisted polymerization and applied for the separation and enrichment of chloramphenicol from honey. The polymers were applied as solid-phase extraction materials to extract and clean up the chloramphenicol in honey, followed by the LC-MS/MS analysis. The morphology of polymers were characterized. The binding property and selectivity of polymers were evaluated and Scatchard analysis was carried out. The polymers appeared to be spherical in shape and have a good selectivity. The apparent maximum binding amount of the polymers was 427.7 μmol g-1. The detection limit of chloramphenicol was 0.13 ng g-1. The linearity range and correlation coefficient of the method were from 0.5 to 100 ng g-1 and 0.999, respectively. The recoveries of six spiked honey samples were in the range of 88%~93 %. The proposed method has been successfully applied for the detection of chloramphenicol from honey.
Template and target information: chloramphenicol
Author keywords: Molecularly imprinted polymers, microwave-assisted polymerization, Solid-phase extraction, Liquid chromatography - tandem mass spectrometry