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Abstract: A chemiluminescence (CL) sensor with high sensitivity and selectivity has been developed for the determination of Ribonuclease A (RNase A). A new material Fe3O4/multi-walled carbon nanotubes/SiO2 (Fe3O4/MWCNTs/SiO2) was introduced into this sensor as a supporting material to prepare a surface molecular imprinting polymer (SMIP). In this work, Fe3O4 not only served as a backbone material in the preparation of the RNase A SMIP, but also as a separation reagent to allow the easy collection of the SMIP complex. Carbon nanotubes and SiO2 were also used as supporting materials to prepare SMIP for their large specific surface area. The Fe3O4/MWCNTs/SiO2 nanocomposite was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques. The adsorption ability of the Fe3O4/MWCNTs/SiO2-SMIP was calculated to be 102 mg g-1, which demonstrated the excellent recognition and adsorption ability of the imprinting cavities situated at or in the proximity of the surface of the Fe3O4/MWCNTs/SiO2. Under optimal conditions, the linear range of the sensor extended from 1.0 x 10-9 mg mL-1 to 1.0 x 10-7 mg mL-1 for RNase A and the detection limit was 3.2 x 10-10 mg mL-1 (3δ ). The proposed sensor was successfully applied for the determination of RNase A in biological samples with recoveries from 93% to 105%
Template and target information: protein, Ribonuclease A, RNas A