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Reference type: Journal
Authors: Khurshid S, Govada L, El-Sharif HF, Reddy SM, Chayen NE
Article Title: Automating the application of smart materials for protein crystallization.
Publication date: 2015
Journal: Acta Crystallographica Section D
Volume: 71
Issue: (3)
Page numbers: 534-540.
DOI: 10.1107/S1399004714027643

Abstract: The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as 'smart materials') for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials
Template and target information: proteins
Author keywords: protein crystallization, Molecularly imprinted polymers, Smart materials


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