MIPs logo MIPdatabase      MIP2024 Conference banner, website is now open, register on site for important updates   
Custom Search
Reference type: Journal
Authors: Altintas Z, Guerreiro A, Piletsky SA, Tothill IE
Article Title: NanoMIP based optical sensor for pharmaceuticals monitoring.
Publication date: 2015
Journal: Sensors and Actuators B: Chemical
Volume: 213
Page numbers: 305-313.
DOI: 10.1016/j.snb.2015.02.043
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0925400515002166

Abstract: Nanoparticles synthesised using molecular imprinting (MIP) with high affinity for diclofenac were produced and applied in the development of an optical sensor based on surface plasmon resonance (SPR). MIPs were synthesised with diclofenac as the template using solid-phase synthesis with photopolymerisation, a method which allows the template to be reused successfully. Particle size of ~132.3 nm 3.2 was achieved with low polydispersity index (0.1), which confirms the quality of the synthesised nanoMIPs. The MIPs were then immobilised on the surface of the SPR-2 gold sensor chips via covalent coupling using EDC/NHS. An affinity based-diclofenac assay was then constructed on the surface of the sensor. The diclofenac drug was then successfully detected in the concentration range of 1.24-80 ng mL-1. The nanoMIP sensor surfaces were then regenerated using glycine-hydrochloric acid solution and reused for subsequent analysis indicating the stability of the sensor surface. Kinetic data analysis indicated a dissociation constant of 1.48 x 10-9 M was achieved. Cross reactivity studies were conducted against other pharmaceuticals using the developed sensor. The nanoMIP affinity towards diclofenac was also confirmed by eluting a diclofenac solution through a solid phase-nanoMIP columns (SPE) and analysing the eluents using an LC-MS. The developed SPR sensor with the nanoMIP showed good potential for using the technology for the capture and detection of diclofenac in water
Template and target information: diclofenac
Author keywords: Molecularly imprinting polymers (MIPs), pharmaceuticals, nanoparticles, biosensor, surface plasmon resonance (SPR)

  Shirt featuring the name Sergey spelled out in symbols of the chemical elements  Periodic table Bag bag  Meerkat somebody mention coffee mug


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner