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Reference type: Journal
Authors: Bates F, del Valle M
Article Title: Voltammetric sensor for theophylline using sol-gel immobilized molecularly imprinted polymer particles.
Publication date: 2015
Journal: Microchimica Acta
Volume: 182
Issue: (5-6)
Page numbers: 933-942.
DOI: 10.1007/s00604-014-1413-4

Abstract: Sensors incorporating molecularly imprinted polymers (MIPs) are feasible in concept though the reproducibility of such devices can be compromised by the large number of interdependent steps. For this reason, many researchers have focused on the synthesis of MIP particles only, not on their immobilization. Herein is presented a sol-gel based method for immobilization of unmodified MIP particles for use in an electrochemical sensor. The macroporous particles were prepared using precipitation-polymerization and imprinted with theophylline. The sol-gel was combined with graphite microparticles (50 μm) and the composite was deposited on the surfaced of an epoxy-graphite electrode. The sensor was then tested for its response to theophylline using differential pulse voltammetry. A limit of detection of 1 μM was observed and a relative standard deviation of 6.85 %. The electrode can be regenerated via a thermal washing process which was accompanied by an initial signal loss of 29.3 %. Any further regeneration caused a signal loss of 2.4 % only
Template and target information: theophylline
Author keywords: molecularly imprinted polymers (MIP), sol-gel, Graphite, theophylline, differential pulse voltammetry


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