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Reference type: Journal
Authors: Chen S, Gan N, Zhang HR, Hu FT, Li TH, Cui H, Cao YT, Jiang QL
Article Title: A portable and antibody-free sandwich assay for determination of chloramphenicol in food based on a personal glucose meter.
Publication date: 2015
Journal: Analytical and Bioanalytical Chemistry
Volume: 407
Issue: (9)
Page numbers: 2499-2507.
DOI: 10.1007/s00216-015-8478-8

Abstract: A portable and antibody-free sandwich assay was fabricated for determination of chloramphenicol (CAP) in animal-derived food by a personal glucose meter (PGM). The sandwich-type strategy was developed on the basis of magnetic molecularly imprinted probe (m-MIP) nanoparticles and a β-cyclodextrin/invertase-functionalized signal tag. Firstly, the m-MIPs were fabricated using polydopamine molecularly imprinted film modified Fe3O4 nanoparticles with 2,2-dichloroacetamide as a template that could capture the 2,2-dichloroacetamide segment of CAP. Secondly, β-cyclodextrin/invertase polymer bioconjungate was synthesized as a signal tag that could recognize the nitrobenzene segment of CAP through host-guest interaction. The dual-specificity recognition model relies on the formation of a sandwich between m-MIPs, different segments of CAP, and the β-cyclodextrin-functionalized signal tag. The sandwich-type complex formed was then subjected to detection with a PGM. The complexes can hydrolyze sucrose to glucose, which can be used for detection with a PGM through invertase. According to our experiment, the concentration of CAP was proportional to the amount of glucose formed, which could quantitatively assess the CAP with a dynamic range of 0.5-50 ng mL-1 and a detection limit of 0.16 ng mL-1 (signal-to-noise ratio of 3). The detection time of the assay was about 1 h, which was obviously shorter than that of competitive ELISA. More importantly, we have successfully applied this on-site assay for CAP screening in animal-derived food
Template and target information: chloramphenicol, CAP
Author keywords: chloramphenicol, Antibody-free, Sandwich-type assay, molecularly imprinted, β-cyclodextrin, Personal glucose meter


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