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Reference type: Journal
Authors: Hu YF, Chen ZH, Fu YJ, He QZ, Jiang L, Zheng JG, Gao YN, Mei PC, Chen ZZ, Ren XQ
Article Title: The amino-terminal structure of human fragile X mental retardation protein obtained using precipitant-immobilized imprinted polymers.
Publication date: 2015
Journal: Nature Communications
Volume: 6
Page numbers: ArticleNo6634.
DOI: 10.1038/ncomms7634

Abstract: Flexibility is an intrinsic property of proteins and essential for their biological functions. However, because of structural flexibility, obtaining high-quality crystals of proteins with heterogeneous conformations remain challenging. Here, we show a novel approach to immobilize traditional precipitants onto molecularly imprinted polymers (MIPs) to facilitate protein crystallization, especially for flexible proteins. By applying this method, high-quality crystals of the flexible N-terminus of human fragile X mental retardation protein are obtained, whose absence causes the most common inherited mental retardation. A novel KH domain and an intermolecular disulfide bond are discovered, and several types of dimers are found in solution, thus providing insights into the function of this protein. Furthermore, the precipitant-immobilized MIPs (piMIPs) successfully facilitate flexible protein crystal formation for five model proteins with increased diffraction resolution. This highlights the potential of piMIPs for the crystallization of flexible proteins
Template and target information: protein

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