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Reference type: Journal
Authors: Gong YR, Niu YL, Gong XH, Ma MH, Ren XW, Zhu WH, Luo RM, Gong BL
Article Title: Preparation of 17β-estradiol-imprinted material by surface-initiated atom transfer radical polymerization and its application.
Publication date: 2015
Journal: Journal of Separation Science
Volume: 38
Issue: (7)
Page numbers: 1254-1261.
DOI: 10.1002/jssc.201401375

Abstract: A novel 17β-estradiol molecularly imprinted polymer was grafted onto the surface of initiator-immobilized silica by surface-initiated atom transfer radical polymerization. The resulting molecularly imprinted polymer was characterized by elemental analysis and thermogravimetric analysis. The binding property of molecularly imprinted polymer for 17β-estradiol was also studied with both static and dynamic methods. The results showed that the molecularly imprinted polymer possessed excellent recognition capacity for 17β-estradiol (180.65 mg/g at 298 K), and also exhibited outstanding selectivity for 17β-estradiol over the other competitive compounds (such as testosterone and progesterone). Then, the determination of trace 17β-estradiol in beef samples was successfully developed by using molecularly imprinted polymer solid-phase extraction coupled with high-performance liquid chromatography. The limit of detection was 0.25 ng/mL, and the amount of 17β-estradiol in beef samples was detected at 2.83 ng/g. This work proposed a sensitive, rapid, reliable, and convenient approach for the determination of trace 17β-estradiol in complicated beef samples
Template and target information: 17β-estradiol, E2
Author keywords: Atom transfer radical polymerization, Beef samples, 17β-estradiol, Molecularly imprinted polymers, Solid-phase extraction

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