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Reference type: Journal
Authors: Hosseini MRM, Hemmati S, Ghaffarinejad A, Motaharian A
Article Title: Fabrication of Molecularly Imprinted Polymer Coated Carbon Nanotubes Modified Gold Electrode for Determination of Cholesterol.
Publication date: 2015
Journal: Analytical & Bioanalytical Electrochemistry
Volume: 7
Issue: (2)
Page numbers: 129-148.
Alternative URL: http://abechem.com/No.%202-2015/2015,7%282%29129-148.pdf

Abstract: A novel molecularly imprinted polymer (MIP) electrochemical sensor for selective detection of cholesterol has been developed by grafting a very thin polymer film on the surface of the functionalized-carbon nanotube (f-CNT) modified gold electrode. This sensor was made by electropolymerization of 2-mercaptobenzimidazole in the presence of cholesterol as a template on the surface of carbon nanotube modified gold electrode. Since cholesterol did not have any electroactivity on MIP/MWCNT/Au electrode in the phosphate buffer; indirect method was used for the determination of cholesterol. In this method, K3Fe(CN)6/K4Fe(CN)6redox couples was used as an electrochemical probe to characterize the sensor using cyclic voltammetry, differential pulse voltammetry and chronoamperometry methods. The linear response range for cholesterol detection was between 2 and 350 mg.dL-1 with a detection limit of 1 mg.dL-1. The sensor exhibited good selectivity for cholesterol, with a satisfactory reproducibility and repeatability. Theproposed electrode was successfully applied for determination of cholesterol in human blood serum.
Template and target information: cholesterol
Author keywords: Cholesterol sensor, molecularly imprinted polymer, Carbon nanotube, electropolymerization, gold electrode


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