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Reference type: Journal
Authors: Li SW, Yang KG, Liu JX, Jiang B, Zhang LH, Zhang YK
Article Title: Surface-Imprinted Nanoparticles Prepared with a His-Tag-Anchored Epitope as the Template.
Publication date: 2015
Journal: Analytical Chemistry
Volume: 87
Issue: (9)
Page numbers: 4617-4620.
DOI: 10.1021/ac5047246

Abstract: The specific recognition of biomolecules by artificial antibodies has inspired fascination among chemists and biologists. Herein, we propose a new method to prepare epitope-oriented surface-imprinted nanoparticles with high template utilization efficiency. Using a His-tag as the anchor to facilitate the epitope immobilization/removal and the self-polymerization of dopamine to control the imprinted shell thickness, the prepared epitope-imprinted nanoparticles show specific recognition of the target protein. Moreover, with improved hydrophilicity of the His-tag-anchored epitope, this method opens up a universal route for imprinting epitopes with various polarities
Template and target information: His-tag, epitope, protein


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