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Reference type: Journal
Authors: Benito-Peņa E, Navarro-Villoslada F, Carrasco S, Jockusch S, Ottaviani MF, Moreno-Bondi MC
Article Title: Experimental Mixture Design as a Tool for the Synthesis of Antimicrobial Selective Molecularly Imprinted Monodisperse Microbeads.
Publication date: 2015
Journal: ACS Applied Materials & Interfaces
Volume: 7
Issue: (20)
Page numbers: 10966-10976.
DOI: 10.1021/acsami.5b02238

Abstract: The effect of the cross-linker on the shape and size of molecular imprinted polymer (MIP) beads prepared by precipitation polymerization has been evaluated using a chemometric approach. Molecularly imprinted microspheres for the selective recognition of fluoroquinolone antimicrobials were prepared in a one-step precipitation polymerization procedure using enrofloxacin (ENR) as the template molecule, methacrylic acid as functional monomer, 2-hydroxyethyl methacrylate as hydrophilic comonomer, and acetonitrile as the porogen. The type and amount of cross-linker, namely ethylene glycol dimethacrylate, divinylbenzene or trimethylolpropane trimethacrylate, to obtain monodispersed MIP spherical beads in the micrometer range was optimized using a simplex lattice design. Particle size and morphology were assessed by scanning electron microscopy, dynamic light scattering, and nitrogen adsorption measurements. Electron paramagnetic resonance spectroscopy in conjunction with a nitroxide as spin probe revealed information about the microviscosity and polarity of the binding sites in imprinted and nonimprinted polymer beads
Template and target information: enrofloxacin, ENR


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