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Reference type: Journal
Authors: Bosserdt M, Erdőssy J, Lautner G, Witt J, Köhler K, Gajovic-Eichelmann N, Yarman A, Wittstock G, Scheller FW, Gyurcsányi RE
Article Title: Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition.
Publication date: 2015
Journal: Biosensors and Bioelectronics
Volume: 73
Page numbers: 123-129.
DOI: 10.1016/j.bios.2015.05.049
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566315301470

Abstract: Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecularly imprinted polymer microarrays on bare gold SPR imaging chips. During electrospotting both the gold chip and the spotting tip are electrically connected to a potentiostat as working and counter electrodes, respectively. The spotting pin encloses the monomer-template protein cocktail that upon contacting the gold surface is in-situ electropolymerized resulting in surface confined polymer spots of ca. 500 -Ám diameter. By repeating this procedure at preprogrammed locations for various composition monomer-template mixtures microarrays of nanometer-thin surface-imprinted films are generated in a controlled manner. We show that the removal and rebinding kinetics of the template and various potential interferents to such microarrays can be monitored in real-time and multiplexed manner by SPR imaging. The proof of principle for microelectrospotting of electrically insulating surface-imprinted films is made by using scopoletin as monomer and ferritin as protein template. It is shown that microelectrospotting in combination with SPR imaging can offer a versatile platform for label-free and enhanced throughput optimization of the molecularly imprinted polymers for protein recognition and for their analytical application
Template and target information: protein, ferritin
Author keywords: Molecularly imprinted polymers, surface imprinting, Electrospotting, Ferritin, Scopoletin, electropolymerization


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