MIPs logo MIPdatabase      MIP2024 Conference banner, website is now open, register on site for important updates   
Custom Search
Reference type: Journal
Authors: Antuña-Jiménez D, Blanco-López MC, Miranda-Ordieres AJ, Lobo-Castañón MJ
Article Title: Artificial enzyme-based catalytic sensor for the electrochemical detection of 5-hydroxyindole-3-acetic acid tumor marker in urine.
Publication date: 2015
Journal: Sensors and Actuators B: Chemical
Volume: 220
Page numbers: 688-694.
DOI: 10.1016/j.snb.2015.05.109

Abstract: A novel approach towards the development of biomimetic electrochemical sensors is presented. It is based on magnetic-core/porous-shell molecularly imprinted composites as recognition phase. The selected substrate is 5-hydroxyindole-3-acetic acid (5-HIAA), an urinary metabolite used as carcinoid tumor maker. Using 5-HIAA as template molecule, hemin, 4-vinylpyridine and ethylenglycoldimethacrylate crosslinker are polymerized onto the magnetic core to obtain an artificial enzyme with peroxidase activity. An aliquot of a suspension of the resulting material is deposited onto a carbon screen printed electrode, and the solid material is fixed with a permanent magnet to prepare the sensor. The product of the catalytic oxidation of 5-HIAA at the sensing phase is monitored by differential pulse voltammetry. This sensor allows the detection of 5-HIAA with good sensitivity (0.72 ± 0.01 nA μM-1) and linear response over a concentration range covering the whole window of normal and pathologic urinary levels of 5-HIAA 1-50 μM (0.2-9.6 ppm) with a detection limit of 1.4 μM (0.27 ppm). Urine samples of healthy individuals, spiked with known concentrations of 5-HIAA, yield good recovery values and reproducibility for normal and pathological concentrations (90-100% recovery values, with RSD below 10%)
Template and target information: 5-hydroxyindole-3-acetic acid, 5-HIAA
Author keywords: artificial enzyme, Molecularly imprinted catalytic polymer, Peroxidase-like catalyst, Core-shell magnetic particles, Electrochemical sensor

  Periodic table Soup - the chemical formula for soup apron  Mug featuring the name Carmen spelled out in the single letter amino acid code  Meerkat somebody mention coffee mug


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner