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Reference type: Journal
Authors: Altintas Z, Gittens M, Guerreiro A, Thompson KA, Walker J, Piletsky S, Tothill IE
Article Title: Detection of Waterborne Viruses Using High Affinity Molecularly Imprinted Polymers.
Publication date: 2015
Journal: Analytical Chemistry
Volume: 87
Issue: (13)
Page numbers: 6801-6807.
DOI: 10.1021/acs.analchem.5b00989

Abstract: Molecularly imprinted polymers (MIPs) are artificial receptor ligands which can recognize and specifically bind to a target molecule. They are more resistant to chemical and biological damage and inactivation than antibodies. Therefore, target specific-MIP nanoparticles are aimed to develop and implemented to biosensors for the detection of biological toxic agents such as viruses, bacteria, and fungi toxins that cause many diseases and death due to the environmental contamination. For the first time, a molecularly imprinted polymer (MIP) targeting the bacteriophage MS2 as the template was investigated using a novel solid-phase synthesis method to obtain the artificial affinity ligand for the detection and removal of waterborne viruses through optical-based sensors. A high affinity between the artificial ligand and the target was found, and a regenerative MIP-based virus detection assay was successfully developed using a new surface plasmon resonance (SPR)-biosensor which provides an alternative technology for the specific detection and removal of waterborne viruses that lead to high disease and death rates all over the world
Template and target information: virus, bacteriophage MS2


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