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Reference type: Journal
Authors: Guo T, Deng QL, Fang GZ, Liu CC, Huang X, Wang S
Article Title: Molecularly imprinted upconversion nanoparticles for highly selective and sensitive sensing of Cytochrome c.
Publication date: 2015
Journal: Biosensors and Bioelectronics
Volume: 74
Page numbers: 498-503.
DOI: 10.1016/j.bios.2015.06.058
Alternative URL: http://www.sciencedirect.com/science/article/pii/S095656631530227X

Abstract: A novel method combined the high selectivity of molecular imprinting technology with the strong fluorescence property of upconversion nanoparticles (UCNPs) for sensing of Cytochrome c (Cyt c) was proposed. The molecularly imprinted material-coated upconversion nanoparticles (UCNPs@MIP) were obtained by in situ coating Cyt c imprinted materials to the surface of the carboxyl modified UCNPs through sol-gel technique. The structure and component of the prepared UCNPs@MIP was investigated by transmission electron microscopy (TEM), power X-ray diffraction (XRD), energy-dispersive X-ray analysis (EDXA) and X-ray photoelectron spectroscopic (XPS). The TEM showed the diameter of UCNPs was 40 nm, and thickness of MIP was 5-10 nm. The fluorescence intensity of UCNPs@MIP reduced gradually with the increase of Cyt c concentration. Under optimum conditions, the imprinting factor is 3.19, and the UCNPs@MIP showed selective recognition for Cyt c among other proteins such as bovine serum albumin (BSA) and Lysozyme (Lyz). Therefore, this new method for sensing protein is very promising for future applications
Template and target information: protein, cytochrome c, Cyt c
Author keywords: molecularly imprinted polymer, Upconversion nanoparticles, Optosensing material, Cytochrome C


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