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Reference type: Journal
Authors: Mishra RK, Hayat A, Catanante G, Istamboulie G, Marty JL
Article Title: Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor.
Publication date: 2016
Journal: Food Chemistry
Volume: 192
Page numbers: 799-804.
DOI: 10.1016/j.foodchem.2015.07.080
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0308814615011139

Abstract: In this work, we propose for the first time a sensitive Ochratoxin A (OTA) detection in cocoa beans using competitive aptasensor by differential pulse voltammetry (DPV). In the proposed method, biotin labeled and free OTA competed to bind with immobilized aptamer onto the surface of a screen printed carbon electrode (SPCE), and percentage binding was calculated. The detection was performed after adding avidin-ALP to perform avidin-biotin reaction; the signal was generated through a suitable substrate 1-naphthyl phosphate (1-NP), for alkaline phosphatase (ALP). The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns specifically designed for OTA. The developed aptasensor showed a good linearity in the range 0.15-5 ng/mL with the limit of detection (LOD) 0.07 ng/mL and 3.7% relative standard deviation (RSD). The aptasensor displayed good recovery values in the range 82.1-85% with 3.87% RSD, thus, demonstrated the efficiency of proposed aptasensor for such matrices
Template and target information: ochratoxin A, OTA
Author keywords: OTA, Cocoa beans, food safety, DPV, Competition based aptasensor


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