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Abstract: An ultrasensitive chemiluminescent biosensor with a high selectivity, based on a bioreceptor surface molecular imprinted polymer (SMIP) that used core-shell Fe3O4@SiO2-multi-walled carbon nanotube nanostructures (Fe3O4@SiO2/MWCNTs) as the backbone material, for bovine hemoglobin (BHb) determination was proposed. The Fe3O4@SiO2/MWCNTs were synthesized with a new method, and then were characterized using SEM, FTIR and XRD techniques. The adsorption ability of Fe3O4@SiO2/MWCNTs-SMIP was evaluated to be 91 mg g-1 following the Langmuir isotherm equation and it demonstrated an excellent recognition and adsorption ability for the imprinted sites located on the surface or near the surface of the Fe3O4@SiO2/MWCNTs. Under optimum conditions of CL, the detection range of BHb was from 5.0 x 10-10 to 7.0 x 10-7 mg mL-1 with a detection limit of 1.5 x 10-10 mg mL-1 (3δ ). The proposed biosensor was successfully applied in the determination of BHb in real samples with high selectivity and sensitivity, and the recoveries were excellent and varied from 92% to 106%. Finally, the possible CL mechanism of the BHb in amplifying the CL signal of the luminol-NaOH-H2O2 system was discussed
Template and target information: protein, bovine hemoglobin, BHb