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Abstract: An electrochemical sensor has been developed for the selective determination of doxorubicin hydrochloride (DOX) using the molecularly imprinted technique. To prepare PEDOT molecularly imprinted polymer (MIP), cyclic voltammetry scans were performed for 7 cycles in the potential range between -0.2 and 1.5 V at 50 mV/s in phosphate buffer solution (pH=7.23) containing 1.0 x 10-5mol/L DOX at room temperature. The MIP electrode was washed by electrochemical method for 4 circles to remove template molecules. Under the optimum conditions,the properties of the electrochemical sensor modified with DOX-imprinted membrane were characterized by cyclic voltammetry(CV),differential pulse voltammetry(DPV) and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was performed after the rebinding reaction of the DOX and the MIP membrane in DOX sample solutions for 20 min. Electrochemical measurements for the sensor were carried out in the supporting electrolyte of 0.005 mol/L K3[Fe(CN)6] solution containing 0.1 mol/L KCl over a potential range of 1.2 to 1.8V, with the pulse amplitude of 50 mV and the scan rate of 50 mV/s. A linear relationship between oxidation peak current and DOX concentration was obtained over the range of 4.0 x 10-7 - 1.0 x 10-6 mol/L with a correlation coefficient of 0.9967 and a detection limit( S/N = 3) of 6.5 x 10-8 mol/L. After regeneration by washing with electrochemical method, the sensor showed excellent reproducibility and good stability. The MIP electrode exhibited very weak response to vinblastine, actinomycin D and daunorubicind, proving a good selectivity. The studied imprinted sensor was successfully applied to the determination of doxorubicin hydrochloride in human blood samples with the relative standard deviation (RSD) below 4% and recovery ranging from 96.0% to 106.7% .
Template and target information: doxorubicin hydrochloride, DOX
Author keywords: molecularly imprinted polymer, Electrochemical sensor, 3,4-ethylenedioxythiophene, doxorubicin hydrochloride