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Reference type: Journal
Authors: Vlatakis G, Andersson LI, Müller R, Mosbach K
Article Title: Drug assay using antibody mimics made by molecular imprinting.
Publication date: 1993
Journal: Nature
Volume: 361
Issue: (6413)
Page numbers: 645-647.
DOI: 10.1038/361645a0

Abstract: LIGAND-BINDING assays are used for determination of minute amounts of substances in the bloodstream. Such assays require a receptor that specifically binds the substance of interest. The receptor used is often an antibody1-5, but antibodies require special handling and a costly production procedure5. We have used molecular imprinting, a method for creating selective recognition sites in synthetic polymers6-8, to prepare polymers that mimic antibody combining sites. Molecular imprints made against theophylline9 and diazepam10 showed strong binding and cross-reactivity profiles similar to those of antibodies. Here we describe a new radiolabelled ligand-binding assay, the molecularly imprinted sorbent assay, which uses antibody mimics. This assay accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique. Antibody mimics, which are stable and readily prepared by molecular imprinting, may provide a useful general alternative to antibodies
Template and target information: theophylline, diazepam

  Mug featuring the name Klaus spelled out in symbols of the chemical elements  Mug featuring the name Lars spelled out in the single letter amino acid code    Mug featuring the template Theophylline


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