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Reference type: Journal
Authors: Ye L, Cormack PAG, Mosbach K
Article Title: Molecularly imprinted monodisperse microspheres for competitive radioassay.
Publication date: 1999
Journal: Analytical Communications
Volume: 36
Issue: (2)
Page numbers: 35-38.
DOI: 10.1039/a809014i

Abstract: In the present study, molecularly imprinted affinity sorbents against theophylline and 17 beta-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micron-scale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the 'classical' particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach


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