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Reference type: Journal
Authors: Taniwaki K, Hyakutake A, Aoki T, Yoshikawa M, Guiver MD, Robertson GP
Article Title: Evaluation of the recognition ability of molecularly imprinted materials by surface plasmon resonance (SPR) spectroscopy.
Publication date: 2003
Journal: Analytica Chimica Acta
Volume: 489
Issue: (2)
Page numbers: 191-198.
DOI: 10.1016/S0003-2670(03)00760-8

Abstract: Polysulfone with an oligopeptide derivative of glutamyl residues (PSf-E-5.8) was evaluated as a candidate material for molecular recognition. PSf-E-5.8 was prepared from the N- carboxyanhydride of gamma-benzyl-L-glutamate (Glu(OBzl)-NCA) initiated by aminomethylated polysulfone (PSf). Films with molecular recognition sites with preference to adenosine (As) were prepared from PSf-E-5.8 by an alternative molecular imprinting method using 9-ethyladenine (9-EA) as a print molecule. The molecular recognition phenomena were studied by surface plasmon resonance (SPR) spectroscopy. The apparent affinity constant of the molecular recognition sites toward As was dependent on the imprinting condition and was increased from 1.30 x 10(4) to 1.60 x 10(4) mol(-1) dm(3) with the decrease in the molecular imprinting ratio from 1.0 to 0.25. From the present study, it was shown that SPR is a convenient and facile method to detect molecular recognition interactions. (C) 2003 Elsevier B.V. All rights reserved


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