MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Kwon OH, Imanishi Y, Ito Y
Article Title: Enhancement of catalytic activity of chemically modified subtilisin Carlsberg in benzene by adjustment of lyophilization conditions.
Publication date: 2000
Journal: Bulletin of the Chemical Society of Japan
Volume: 73
Issue: (5)
Page numbers: 1277-1282.
DOI: 10.1246/bcsj.73.1277

Abstract: Alteration of the lyophilization conditions significantly enhanced not only the activity of insoluble native subtilisin Carlsberg but also that of solubilized enzyme in benzene. The catalytic activity (k(cat)/K-m) for the transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with l- propanol in benzene was enhanced more than 1000 times by the solubilization (100-fold enhancement) and the adjustment of lyophilization conditions (10-fold enhancement). Subtilisin Carlsberg was modified with polyethylene glycol (PEG-Sub) so that it could be solubilized in organic solvents. The effects of pH of the aqueous enzyme solution, of salts, or of a substrate analog in the aqueous solution before the lyophilization process, on the catalytic activity of native subtilisin and PEG-Sub were investigated. The activities of both the native subtilisin and PEG-Sub depended on the pH. The activities of both PEG-Sub and the native subtilisin were enhanced by the presence of salts. The presence of a substrate analog, N-Ac-L-Phe, also enhanced the activity of the native subtilisin and PEG-Sub. These results demonstrated that the organic solvent-solubilized subtilisin was affected by the lyophilization conditions

  I love MIPs shirt  multi MIPs logo mug  Ireland periodic table shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner