MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Raitman OA, Chegel VI, Kharitonov AB, Zayats M, Katz E, Willner I
Article Title: Analysis of NAD(P)+ and NAD(P)H cofactors by means of imprinted polymers associated with Au surfaces: A surface plasmon resonance study.
Publication date: 2004
Journal: Analytica Chimica Acta
Volume: 504
Issue: (1)
Page numbers: 101-111.
DOI: 10.1016/S0003-2670(03)00511-7

Abstract: Crosslinked films consisting of the acrylamide- acrylamidophenylboronic acid copolymer that are imprinted with recognition sites for beta-nicotinamide adenine dinucleotide (NAD(+)), beta-nicotinamide adenine dinucleotide phosphate NADP(+), and their reduced forms (NAD(P)H), are assembled on Au-coated glass supports. The binding of the oxidized cofactors NAD(+) or NADP(+) or the reduced cofactors NADH or NADPH to the respective imprinted sites results in the swelling of the polymer films through the uptake of water. Surface plasmon resonance (SPR) spectroscopy is employed to follow the binding of the different cofactors to the respective imprinted sites. The imprinted recognition sites reveal selectivity towards the association of the imprinted cofactors. The method enables the analysis of the NAD(P)(+) and NAD(P)H cofactors in the concentration range of 1 x 10(-6) to 1 x 10(-3) M. The cofactor-imprinted films associated with the Au-coated glass supports act as active interfaces for the characterization of biocatalyzed transformations that involve the cofactor- dependent enzymes. This is exemplified with the characterization of the biocatalyzed oxidation of lactate to pyruvate in the presence of NAD(+) and lactate dehydrogenase using the NADH-imprinted polymer film. (C) 2003 Elsevier B.V. All rights reserved

  I love MIPs magnet  multi MIPs logo mug  Irish periodic table patriotic shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner