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Reference type: Journal
Authors: Chirica G, Remcho VT
Article Title: Silicate entrapped columns - new columns designed for capillary electrochromatography.
Publication date: 1999
Journal: Electrophoresis
Volume: 20
Issue: (1)
Page numbers: 50-56.
DOI: 10.1002/(SICI)1522-2683(19990101)20:1<50::AID-ELPS50>3.0.CO;2-O
Alternative URL: http://www.chem.orst.edu/remcho/publications/electrophoresis20.pdf

Abstract: Designed especially for capillary electrochromatography (CEC), silicate-entrapped columns are made by trapping particles of chromatographic packing material in a network of silica. Once entrapped, the capillary no longer requires frits. This renders a more homogeneous and stable packed bed. Accidental breakage of the fragile frits is not an issue with these robust columns. Columns packed with reverse-phase material subjected to silicate entrapment demonstrated faster separations of retained analytes and increased efficiencies compared with nonentrapped columns. The method was also used to prepare chiral CEC columns by entrapping a molecular imprinted polymeric (MIP) packing having minimal surface charge density, thus being unable alone to support sufficient electroosmotic flow for CEC


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