Abstract: Molecular imprinted polymers (MIP) by using single templates (pentoxifylline, caffeine and theophylline) and mixed-templates (pentoxifylline-theophylline pentoxifylline-caffeine, and caffeine-theophylline) were proposed in this paper. The MIP were prepared using MAA as the monomer, EGDMA as the crosslinker and AIBN as the initiator. The obtained polymer particles (particle size was about 25-35 µm) was packed into HPLC column (3.9×150 mm). The result of the experiment showed that the retention factors and resolutions of two mixed-template imprinting polymers were higher than that of single template imprinting polymers. This indicated increase the affinity of the MIP. The improved affinity on the mixed-template MIP was regarded as a result of cooperation effect of the binding sites.