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Reference type: Book Chapter
Authors: Wulff G
Publication date: 1996
Chapter title: Models of the binding sites of enzymes: Template induced preparation of specific binding sites in crosslinked polymers.
Chapter number: 31
Page numbers: 639-649.
DOI: 10.1016/S1569-2558(08)60143-5 Alternative URL: http://www.sciencedirect.com/science/article/B7CTF-4SMFKMJ-13/1/647fa7d32ae3ce854ba823292c134d44

Book title: Biochemical Technology Part B
Editors: Bittar EE, Danielsson B, Bulow L
Publisher: Elsevier
City: Amsterdam
Series title: Advances in Molecular and Cell Biology
Volume number: 15B

Abstract: Various attempts to produce specific binding sites in synthetic polymers analogous to those of biological receptors or natural enzymes are described here. To this end, an imprinting procedure was used with the aid of templates in cross-linked polymers. Suitable polymerizable binding groups were bound to a template molecule. This complex was copolymerized into highly cross-linked polymers. After the removal of the templates the remaining cavities possessed a shape and arrangement of their functional groups corresponding to that of the template. New developments in the application of this technique and experiments towards the understanding of the mechanism of the molecular recognition are discussed in detail.

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