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Authors: Meng ZH, Yamazaki T, Sode K
Article Title: Construction of artificial cyclic amide amidohydrolases using molecular imprinting technique.
Publication date: 2005
Journal: Biotechnology Letters
Volume: 27
Issue: (7)
Page numbers: 471-475.
DOI: 10.1007/s10529-005-2223-x
Abstract: A general molecular imprinting approach is proposed to synthesize artificial enzymes to mimic the family of cyclic amide amidohydrolases which share similar active site and catalytic mechanism. The artificial enzymes were constructed by co-polymerizing 4(5)-vinylimidazole-Co2+-methacrylic acid clusters with divinylbenzene micro-spheres in the presence of corresponding substrates. The artificial enzymes mimicked creatininase and hydantoinase by showing specific affinity towards the corresponding substrates in buffer. The artificial hydantoinase also showed specific affinity towards corresponding substrate in organic solvent, and catalyzed the hydrolysis of hydantoin
Template and target information: creatinin, hydantoin
Author keywords: artificial enzyme, creatininase, cyclic amide amidohydrolase, hydantoinase, molecular imprinting
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