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Reference type: Journal
Authors: Chen Y, Brazier J, Yan M, Prahl SA
Article Title: Steady-State Fluorescence Anisotropy Studies Of Molecularly Imprinted Polymer Sensors.
Publication date: 2004
Journal: Proceedings of the Oregon Academy of Science
Volume: 40
Page numbers: 49.
Alternative URL: http://www.bme.ogi.edu/~prahl/pubs/abs/chen04a.html

Abstract: Molecularly imprinted polymers (MIPs) are used as recognition elements in biochemical sensors. In a fluorescence-based MIP sensor system, it can be difficult to distinguish the analyte fluorescence from the fluorescence of the polymer itself. We studied steady-state fluorescence anisotropy of anthracene imprinted in a polymer (polyurethane) matrix. Vertically polarized excitation light was incident on MIP films coated on silicon wafers; vertically and horizontally polarized emission was measured. We compared the fluorescence anisotropy of MIPs with imprinted molecules, MIPs with the imprinted molecules extracted, MIPs with rebound molecules, and non-imprinted control polymers. It is shown that differences in fluorescence anisotropy between the polymers and imprinted fluorescent molecules may provide a means to discriminate the fluorescence of analyte from that of the background polymer.


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