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Reference type: Journal
Authors: Gao SH, Wang W, Wang BH
Article Title: Building fluorescent sensors for carbohydrates using template-directed polymerizations.
Publication date: 2001
Journal: Bioorganic Chemistry
Volume: 29
Issue: (5)
Page numbers: 308-320.
DOI: 10.1006/bioo.2001.1219

Abstract: The ability to custom-make fluorescent sensors for different analytes could have a tremendous impact in a variety of areas. Template-directed polymerization or molecular imprinting seems to be a promising approach for the preparation of high-affinity and specific binding sites for different template molecules. However, the application of molecular imprinting in the preparation of fluorescent sensors has been hampered by the lack of suitable fluorescent tags, which would respond to the binding event with significant fluorescence intensity changes. We have designed and synthesized a fluorescent monomer (1) that allows for the preparation of fluorescent sensors of cis diols using molecular imprinting methods. This monomer has been used for the preparation of imprinted polymers as sensitive fluorescent sensors for D-fructose. The imprinted polymers prepared showed significant fluorescence intensity enhancement upon binding with the template carbohydrate. t (C) 2001 Academic Press

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