MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Harvey SD, Mong GM, Ozanich RM, Mclean JS, Goodwin SM, Valentine NB, Fredrickson JK
Article Title: Preparation and evaluation of spore-specific affinity-augmented bio-imprinted beads.
Publication date: 2006
Journal: Analytical and Bioanalytical Chemistry
Volume: 386
Issue: (2)
Page numbers: 211-219.
DOI: 10.1007/s00216-006-0622-z

Abstract: A novel, affinity-augmented, bacterial spore-imprinted, bead material was synthesized, based on a procedure developed for vegetative bacteria. The imprinted beads were intended as a front-end spore capture/concentration stage of an integrated biological detection system. Our approach involved embedding bead surfaces with Bacillus thuringiensis kurstaki (Bt) spores (as a surrogate for Bacillus anthracis) during synthesis. Subsequent steps involved lithographic deactivation using a perfluoroether; spore removal to create imprint sites; and coating imprints with the lectin, concanavalin A, to provide general affinity. The synthesis of the intended material with the desired imprints was verified by scanning electron and confocal laser-scanning microscopy. The material was evaluated using spore-binding assays with either Bt or Bacillus subtilis (Bs) spores. The binding assays indicated strong spore-binding capability and a robust imprinting effect that accounted for 25% additional binding over non-imprinted controls. The binding assay results also indicated that further refinement of the surface deactivation procedure would enhance the performance of the imprinted substrate
Template and target information: bacterial spores, Bacillus thuringiensis kurstaki spores
Author keywords: Bio-imprinted beads, Selective spore capture, concentration, Analysis of biological pathogens

  Mister Benzene shirt  Chemistry peptide mug  Beach bunny periodic table shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner