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Reference type: Journal
Authors: Yan H, Row KH
Article Title: Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of tryptophan and N-CBZ-phenylalanine enantiomers.
Publication date: 2006
Journal: Biotechnology and Bioprocess Engineering
Volume: 11
Issue: (4)
Page numbers: 357-363.
DOI: 10.1007/BF03026253
Alternative URL: http://bbe.or.kr/home/journal/include/downloadPdf.asp?articleuid={6177D10E-900F-4BA8-83C6-0C29DBFB58F1}

Abstract: Monolithic molecularly imprinted columns were designed and prepared by an in-situ thermal-initiated copolymerization technique for rapid separation of tryptophan and N-CBZ-phenylalanine enantiomers. The influence of polymerization conditions and separation conditions on the specific molecular recognition ability for enantiomers and diastereomers was investigated. The specious molecular recognition was found to be dependent on the stereo structures and the arrangement of functional groups of the imprinted molecule and the cavities in the molecularly imprinted polymer (MIP). Moreover, hydrogen bonding interactions and hydrophobic interactions played an important role in the retention and separation. Compared to conventional MIP preparation procedures, the present method is very simple, and its macroporous structure has excellent separation properties. ®KSBB
Template and target information: tryptophan, N-CBZ-phenylalanine
Author keywords: molecular imprinted polymer, monolithic column, N-CBZ-phenylalanine, special molecular recognition, Tryptophan


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