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Reference type: Journal
Authors: Kublickas R, Werner C, Jariene G, Voit B, Lasas L
Article Title: Polyacrylamide gels containing ionized functional groups for the molecular imprinting of human growth hormone.
Publication date: 2007
Journal: Polymer Bulletin
Volume: 58
Issue: (3)
Page numbers: 611-617.
DOI: 10.1007/s00289-006-0694-0
Alternative URL: http://www.springerlink.com/content/n15816686655v838/

Abstract: Networks of polyacrylamide having methacrylic acid and 2-(dimethylamino)ethyl methacrylate incorporated were studied for the possibility of imprinting human growth hormone. Two templates the monomeric and the dimeric molecular forms of human growth hormone were for the first time imprinted using a hydrogel containing charged functional groups. The results show that approximately 90% (w/w) of the templates could be extracted from the molecularly imprinted acrylamide polymers. The molecularly imprinted polymer adsorbed approximately 70 times (for the monomeric form of the hormone) and 60 times (for the dimeric form) more of the template than of the non-imprinted polymer. The selectivity of the molecularly imprinted polymers was also studied.
Template and target information: peptide, human growth hormone, monomeric form of human growth hormone, dimeric form of human growth hormone

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