Abstract: 4-aminopyridine molecularly imprinted polymer based monolithic capillary column was prepared by a in situ polymerization process in fused silica capillary column. Based on the comparison of the influence of acetonitrile content on the migration behavior of 4 aminopyridine and 2 aminopyridine on non imprinted polymer based column and silanized open tubular column, the mechanism of the separation on the 4 aminopyridine molecularly imprinted polymer based column by capillary electrochromatography was discussed. The recognition ability of the 4 aminopyridine molecular imprinted polymer was affected by the acetonitrile content so strongly that the elution order of the solutes might be reversed. When the acetonitrile content was high, the chromatographic retention played a main role in the migration process; when the concentration of acetonitrile was low, electrophoretic migration played a main role.
Template and target information: 4-aminopyridine