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Reference type: Journal
Authors: Zhao KY, Cheng GX, Huang JJ, Ying XG
Article Title: Rebinding and recognition properties of protein-macromolecularly imprinted calcium phosphate/alginate hybrid polymer microspheres.
Publication date: 2008
Journal: Reactive and Functional Polymers
Volume: 68
Issue: (3)
Page numbers: 732-741.
DOI: 10.1016/j.reactfunctpolym.2007.11.011
Alternative URL: http://www.sciencedirect.com/science/article/B6TY0-4R5VYV5-1/2/a5a3f33b18338971dc3e9f285c0d9bbe

Abstract: Calcium phosphate/alginate hybrid polymer microspheres with bovine serum albumin (BSA) embedded and coated on the surface were prepared with sodium alginate (SA) and (NH4)2HPO4 by using CaCl2 as the gelling agent in an inverse suspension. The morphology and pore structure of imprinted hybrid polymer microspheres was observed by optical microscopy, ESEM, TEM and SEM. Infrared spectrum analysis demonstrated that new hybrid components were produced in the polymer microspheres. Rebinding dynamic and thermodynamic behaviors of the two imprinted microspheres were evaluated, resulting in a higher BSA affinity for imprinted microspheres relative to non-imprinted ones. The factors influencing the imprinting efficiency (IE) of imprinted microspheres were also studied, including the concentration of CaCl2, template content and pH in rebinding solution, etc. All the results indicated that the surface imprinted microspheres exhibited better imprinting efficiency and higher rebinding capacity. Selectivity tests showed that the imprinted beads exhibited good recognition properties for the template protein versus others
Author keywords: phosphate, alginate, Hybrid polymer microspheres, surface molecular imprinting, Imprinting efficiency (IE), Recognition


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