MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Claude B, Morin P, Lafosse M, Belmont AS, Haupt K
Article Title: Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer.
Publication date: 2008
Journal: Talanta
Volume: 75
Issue: (2)
Page numbers: 344-350.
DOI: 10.1016/j.talanta.2007.11.037
Alternative URL: http://www.sciencedirect.com/science/article/B6THP-4R5VYN6-1/2/992b11e4059b6c0c2ed41be75676725d

Abstract: A molecularly imprinted polymer (MIP) has been prepared by a thermal polymerisation method using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linking agent, chloroform as porogenic solvent and an oleanane triterpene compound (18-β-glycyrrhetinic acid) as imprinted molecule (template). Equilibrium ligand binding experiments were done to assess the performance of the MIP relative to non-imprinted polymer (NIP). After optimisation of SPE protocol (CHCl3 as washing solvent and MeOH as elution solvent), successful imprinting was confirmed by comparison of the recoveries between NIP (5%) and MIP (97%) cartridges. The binding capacity of the MIP for 18-β-glycyrrhetinic acid was determined to be 0.94 mg g-1. Four structurally related oleanane triterpenes (18-α-glycyrrhetinic acid, oleanolic acid, echinocystic acid, erythrodiol) were selected to assess the MIP selectivity. Experimental data illustrated the influence of functional groups on the triterpene skeleton. The MIP was applied to the solid-phase extraction of triterpenoids from a plant extract prior HPLC analysis. However, CHCl3 was replaced by ACN during the washing step in order to suppress non-specific interactions due to polar matrix components. A selective extraction of 18-β-glycyrrhetinic acid from hydrolyzed extract of liquorice roots was achieved with a good extraction yield (98%)
Author keywords: molecularly imprinted polymer, Triterpene, Plant extract, Glycyrrhetinic acid, Natural compounds


  Mug featuring the name Berengere spelled out in symbols of the chemical elements  Mug featuring the name Philippe spelled out in the single letter amino acid code  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner