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Reference type: Journal
Authors: Qin L, He XW, Li WY, Zhang YK
Article Title: Molecularly imprinted polymer prepared with bonded β-cyclodextrin and acrylamide on functionalized silica gel for selective recognition of tryptophan in aqueous media.
Publication date: 2008
Journal: Journal of Chromatography A
Volume: 1187
Issue: (1-2)
Page numbers: 94-102.
DOI: 10.1016/j.chroma.2008.02.004
Alternative URL: http://teresacasimiro.com.sapo.pt/Bibliographic_References/8.pdf

Abstract: A novel molecularly imprinted polymer (MIP) selective for tryptophan (Trp) was described where polymerization was performed in aqueous media. Three kinds of molecularly imprinted polymers were prepared with surface molecular imprinting technique on functionalized silica gel (F-silica gel). MIPs prepared using bonded β-cyclodextrin (β-CD) and acrylamide (AA), either separately or in combination have shown various recognition properties. The results of adsorption experiments indicated that the selectivity of MIP, which was synthesized with bonded β-CD and AA [MIP(1)], was superior to those obtained with AA [MIP(2)] or bonded β-CD [MIP(3)]. In addition, the high-performance liquid chromatography (HPLC) column packed with MIP(1) could not only separate Trp from other aromatic amino acids, but also separate the template from its enantiomer in aqueous mobile phase. This study developed a new method for chiral amino acid separation and purification
Template and target information: tryptophan, Trp
Author keywords: Surface molecular imprinting technique, silica gel, β-cyclodextrin, Tryptophan, high-performance liquid chromatography, enantiomer separation


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