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Reference type: Journal
Authors: Skottrup PD, Nicolaisen M, Justesen AF
Article Title: Towards on-site pathogen detection using antibody-based sensors.
Publication date: 2008
Journal: Biosensors and Bioelectronics
Volume: 24
Issue: (3)
Page numbers: 339-348.
DOI: 10.1016/j.bios.2008.06.045
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4SXH9X2-5/2/8cf5fccdbc86230fbd69c080fda79711

Abstract: In this paper, the recent progress within biosensors for plant pathogen detection will be reviewed. Bio-recognition layers on sensors can be designed in various ways, however the most popular approach is to immobilise antibodies for specific capture of analytes. Focus will be put on antibody surface-immobilisation strategies as well as the use of antibodies in the widely used sensors, quartz crystal microbalance, surface plasmon resonance and cantilevers. We will describe the available data on antibody-based plant pathogen detection and furthermore use examples from detection of the pathogens Salmonella, Listeria monocytogenes, Streptococcus mutans, Bacillus cereus, Bacillus anthracis, Campylobacter and Escherichia coli. We will touch upon optimal assay design and further discuss the strengths and limitations of current sensor technologies for detection of viruses, bacteria and fungi
Template and target information: Review - plant pathogen detection
Author keywords: immunosensor, Pathogen, Agriculture, mycotoxin, Point-of-care diagnostics, biomolecular interaction, Molecularly imprinted polymers, antibody, Real-time PCR, SPR, QCM, Cantilever, Salmonella, Listeria monocytogenes, Streptococcus mutans, Bacillus cereus, Bacillus anthracis, Campylobacter, Escherichia coli

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