MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Cabirol FL, Tan PL, Tay B, Cheng S, Hanefeld U, Sheldon RA
Article Title: Linum usitatissimum Hydroxynitrile Lyase Cross-Linked Enzyme Aggregates: A Recyclable Enantioselective Catalyst.
Publication date: 2008
Journal: Advanced Synthesis & Catalysis
Volume: 350
Issue: (14-15)
Page numbers: 2329-2338.
DOI: 10.1002/adsc.200800309

Abstract: An immobilized form of the hydroxynitrile lyase from Linum usitatissimum (LuHNL) as cross-linked enzyme aggregate (CLEA) with high specific activity (303.5 U/g) and recovery (33%) was developed. Molecular imprinting using 2-butanone as additive in the immobilization process improved the synthetic activity of the biocatalyst. LuCLEA could be partially recycled for the synthesis of (R)-2-butanone cyanohydrin on a preparative scale over two batches. The enantioenriched cyanohydrin obtained was further hydrolyzed to give (R)-2-hydroxy-2-methylbutyric acid in 85% yield (from 2-butanone) and 87% ee
Template and target information: 2-butanone
Author keywords: biocatalysis, cross-linked enzyme aggregate (CLEA), cyanohydrins, hydroxynitrile lyase, oxynitrilase, quaternary stereogenic carbon


  mipdatabase.com logo special offer joke shirt  Lab Hunk Periodic shirt  HE-MAN polymer chemistry shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner