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Reference type: Journal
Authors: Cabirol FL, Tan PL, Tay B, Cheng S, Hanefeld U, Sheldon RA
Article Title: Linum usitatissimum Hydroxynitrile Lyase Cross-Linked Enzyme Aggregates: A Recyclable Enantioselective Catalyst.
Publication date: 2008
Journal: Advanced Synthesis & Catalysis
Volume: 350
Issue: (14-15)
Page numbers: 2329-2338.
DOI: 10.1002/adsc.200800309

Abstract: An immobilized form of the hydroxynitrile lyase from Linum usitatissimum (LuHNL) as cross-linked enzyme aggregate (CLEA) with high specific activity (303.5 U/g) and recovery (33%) was developed. Molecular imprinting using 2-butanone as additive in the immobilization process improved the synthetic activity of the biocatalyst. LuCLEA could be partially recycled for the synthesis of (R)-2-butanone cyanohydrin on a preparative scale over two batches. The enantioenriched cyanohydrin obtained was further hydrolyzed to give (R)-2-hydroxy-2-methylbutyric acid in 85% yield (from 2-butanone) and 87% ee
Template and target information: 2-butanone
Author keywords: biocatalysis, cross-linked enzyme aggregate (CLEA), cyanohydrins, hydroxynitrile lyase, oxynitrilase, quaternary stereogenic carbon

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